Research in depth

Pong, learning, and sentience: three different claims.

Better performance can be interesting without answering the biggest question. What matters is which claim an experimental setup can actually test.

What claim does each statement make?

The DishBrain paper’s title links playing a game with learning and sentience. In a laboratory, these words need to be tied to a setup and observable criteria. For this cell experiment, it helps to separate three levels.

Claim and required evidence
ClaimWhat needs to be checkedWhat does not yet follow
Cell activity influences PongHow signals are recorded and translated into paddle movement.That the culture perceives a screen or understands the rules of the game.
Performance changesWhich measure is recorded, over what period, and against which control.That every change demonstrates a general capacity for learning.
The culture is sentient (as the paper’s title says)Which definition is used, and suitable criteria for it.That an improvement in a game performance measure shows feeling or subjective experience.

The technical environment is part of the system being studied

The setup translates ball positions into stimuli and cell activity into paddle movement. The feedback is also specified. The observed behaviour therefore arises within a coupling between biological culture and technical environment. Summarising it only as “cells play Pong” would lose sight of these conditions. [W17]

An appropriate question for such experiments is: what change occurs under this feedback, and how does it differ from comparison conditions? An interpretation beyond that requires further arguments. The interactive experiment diagram shows this technical loop.

What scholarly criticism contributes

Balci and other authors challenge the far-reaching terminology and conclusions of the original paper. Their criticism concerns mainly the interpretation, but it also questions some results and their controls. A commentary is a different contribution from a new study with its own data. [N1]

We read the submitted manuscript archived with HAL. We do not silently equate it with every formulation in the published final version, nor claim to have assessed the complete exchange including all responses.

How we examine a far-reaching headline

  1. Describe the observed process in our own words, as narrowly as possible.
  2. Find the measured quantity and its comparison condition.
  3. Check whether the strong term is defined and tested in the experiment.
  4. Present alternative explanations and scholarly objections separately from the result.
  5. Limit the conclusion to what this evidence supports.

This is not a new experimental refutation of the DishBrain paper. It is a transparent way to write an explanation without mixing up the setup, result, and interpretation. How we work applies this distinction to other study checks too.

Sources for this in-depth explanation

W17

In vitro neurons learn and exhibit sentience when embodied in a simulated game-world

Kagan et al. 2022, Neuron, DishBrain, 10.1016/j.neuron.2022.09.001

Study or document
Neuronal cultures on a microelectrode array with closed-loop feedback to a simulation
Finding
The authors report improved performance measures within Pong sessions.
Evidence location
DishBrain system architecture and results on performance under feedback
Limitation
Measurement, interpretation as learning, and a claim about sentience are different levels. Criticism is included as N1.

Full text (introduction, figure legends, discussion at the relevant evidence locations) · DOI: 10.1016/j.neuron.2022.09.001 · Checked 14 October 2026

N1

A response to claims of emergent intelligence and sentience in a dish

Balci et al. 2023, Neuron 111(5):604-605, 10.1016/j.neuron.2023.02.009 (HAL manuscript, submittedVersion)

Study or document
Scholarly commentary, not a new experimental study
Finding
The authors criticise the interpretation and terminology in the DishBrain paper and question the strength of some results.
Evidence location
HAL manuscript, paragraphs on sentience, intelligence, and generalising the findings
Limitation
The submittedVersion was read. This text alone does not represent the complete scientific exchange.

Scholarly commentary; HAL manuscript read (submittedVersion) · DOI: 10.1016/j.neuron.2023.02.009 · Checked 14 October 2026